NAD+ (Nicotinamide Adenine Dinucleotide)
Critical molecule for cellular energy and DNA repair. Levels may drop ~50% by middle age. Activates sirtuin longevity genes and powers PARP DNA repair enzymes. Injectable form bypasses oral absorption issues.
Key Facts
- ClassificationNAD+ is a critical coenzyme in redox reactions and substrate for sirtuins, PARPs, and CD38.
- Primary BenefitsLongevity (10/10) · Brain & Cognition (7/10) · Sleep (5/10)
- AdministrationInjection
- Typical Dose25-50 mg subq, 3 times a week on an empty stomach. Start at 25 mg. Can be mixed in the same syringe with SS-31. Flushing (warm, red skin) is common if you go above 100 mg.
- Evidence StrengthModerate — 20 peer-reviewed studies referenced · Community sentiment 9/10
- Stacks Well WithSS-31, MOTS-c, Epithalon, Semax
- Legal StatusResearch Use Only · not prescribable in US
Quick Facts
- From price
- $75
- Type
- molecule
- Administration
- Injection
- Evidence
- moderate
- Studies referenced
- 20
- Community sentiment
- 9/10
- Stacks with
- 7 peptides
- Regulatory
- Research Use Only
Top benefits
Week 1–2
Increased energy and mental clarity, often noticeable within hours of IV infusion. Improved exercise recovery. Possible flu-like symptoms during infusion.
Week 3–6
Sustained energy improvements. Better mitochondrial function reflected in endurance and recovery. Improved sleep quality. Skin appearance may improve.
Week 8+
Cumulative cellular repair benefits. Improved metabolic biomarkers. Sustained cognitive clarity. DNA repair capacity enhanced. Anti-aging effects building.
Common Side Effects
Scientific Overview
NAD+ is a critical coenzyme in redox reactions and substrate for sirtuins, PARPs, and CD38. Intracellular levels may decline substantially between ages 40-60. Exogenous supplementation bypasses the NAMPT rate-limiting step, improving mitochondrial membrane potential, energy production, and DNA repair capacity.
Dosing
25-50 mg subq, 3 times a week on an empty stomach. Start at 25 mg. Can be mixed in the same syringe with SS-31. Flushing (warm, red skin) is common if you go above 100 mg.
Reconstitution, storage, injection sites & timing
Benefit Profile
| Vendor | Variant | Price | |
|---|---|---|---|
| Limitless Biotech | 500mg | $74.99BEST | Code: PSTACK10Buy |
| Peptide Sciences ✕ Announced voluntary shutdown — stock availability may be limited or unavailable. Pricing data may be outdated. | 500mg | $85.00 | Out of stock |
Stacks Well With
SS-31 (Elamipretide)
Engineered to target inner mitochondrial membrane via cardiolipin binding. Stabilizes cristae, improves ATP production, and reduces oxidative stress. FDA-approved for Barth syndrome (as Forzinity) — the first FDA-approved mitochondria-targeted therapy.
MOTS-c
Mitochondrial DNA-encoded peptide that activates AMPK, improving insulin sensitivity, fat burning, and mimicking exercise at the cellular level. Stacks well with SS-31 and NAD+.
Epithalon (Epitalon)
Longevity peptide from the pineal gland. Activates telomerase to protect DNA caps and restores melatonin production. Russian studies showed 11-16% lifespan increase in animals.
Semax
Brain-boosting peptide that increases BDNF by ~1.4x at the protein level and up to 3x at mRNA level in animal studies. Promotes neurogenesis, improves cerebral blood flow. Used clinically in Russia for stroke recovery and cognitive decline.
Selank
Russian anxiolytic peptide working on GABA, serotonin, and enkephalin systems. Eases anxiety without sedation or dependence risk. Also boosts immunity. Decades of clinical use.
Retatrutide
Next-gen triple agonist from Eli Lilly hitting GLP-1, GIP, and glucagon receptors. Phase 2 showed 24.2% weight loss at 48 weeks — highest of any incretin therapy. At low doses, optimizes metabolism without major weight loss.
Dihexa
Brain peptide originally claimed to be 10 million times more potent than BDNF for forming connections. However, the foundational paper was retracted in April 2025 after confirmed data fabrication. A clinical trial also failed all endpoints. Evidence base is now significantly weakened.
Related ⏳ Longevity Peptides
Other peptides strong in longevity
Destroys senescent "zombie cells" that cause aging and inflammation.
Thymalin8/10Restores immune function from thymus gland extract. Anti-aging effects.
Humanin8/10Protective mitochondrial peptide that shields cells from oxidative damage.
GHK-Cu (Copper Peptide)7/10Copper peptide that rebuilds collagen, tightens skin, and heals wounds.
Vesugen6/10Cardiovascular bioregulator that strengthens blood vessels.
SLU-PP-3326/10Exercise mimetic that activates endurance pathways without working out.